ISOC2 Antibody / Isochorismatase domain-containing protein 2 (FY12695)

Immunofluorescent staining of ISOC2 using anti-ISOC2 antibody (green). ISOC2 was detected in an immunocytochemical section of BEAS-2B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-ISOC2 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of ISOC2 using anti-ISOC2 antibody. Lane 1: human LNCAP whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISOC2 antibody at 0.25 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for ISOC2 at approximately 22 kDa. The expected molecular weight of ISOC2 is ~22 kDa.