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Home >> Antibodies >> Interleukin-1 beta Antibody

Interleukin-1 beta Antibody [clone IL1B/4650] (V4913)

  Catalog No Formulation Size Price (USD)  
Image V4913-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4913-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4913SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using Interleukin-1 beta antibody (clone IL1B/4650). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.
IHC staining of FFPE human adrenal gland tissue with Interleukin-1 beta antibody (clone IL1B/4650). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Interleukin-1 beta antibody (clone IL1B/4650) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name IL1B/4650
Purity Protein A/G affinity
UniProt P01584
Localization Cytoplasm, Extracellular (secreted)
Applications ELISA (Order BSA-free format for coating) :
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Interleukin-1 beta antibody is available for research use only.
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Description

Recognizes a protein of 17-31kDa, identified as interleukin 1beta. It shows no cross-reaction with interleukin 1alpha. Epitopes of mAb IL1B/463 and IL1B/4650 are different and provide a good pair for developing an ELISA. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Application Notes

Optimal dilution of the Interleukin-1 beta antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 100-269) from the human protein was used as the immunogen for the Interleukin-1 beta antibody.

Storage

Aliquot the Interleukin-1 beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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