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Home >> Antibodies >> IL17A Antibody

IL17A Antibody (RQ4021)

  Catalog No Formulation Size Price (USD)  
Image RQ4021 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC testing of FFPE mouse spleen tissue with IL17A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat spleen tissue with IL17A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat pancreas tissue with IL17A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of human Jurkat cell lysate with IL17A antibody at 0.5ug/ml. Expected molecular weight: 15-20 kDa (monomer), 30-40 kDa (homodimer), depending on glycosylation level.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q62386
Localization Secreted, cytoplasmic
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
ELISA (Capture) : 0.1-0.5ug/ml
Limitations This IL17A antibody is available for research use only.
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Description

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Application Notes

Optimal dilution of the IL17A antibody should be determined by the researcher.

Immunogen

A recombinant mouse partial protein corresponding to amino acids A26-A158 was used as the immunogen for the IL17A antibody.

Storage

After reconstitution, the IL17A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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