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Home >> Antibodies >> IL-6 Antibody

IL-6 Antibody (R30787)

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  Catalog No Formulation Size Price (USD)  
Image R30787 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of IL-6 antibody and recombinant human protein: (1) 10ng, (2) 5ng, (3) 2.5ng
Western blot testing of IL-6 antibody and recombinant human protein: (1) 10ng, (2) 5ng, (3) 2.5ng
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P05231
Applications Western blot : 0.5-1ug/ml
Limitations This IL-6 antibody is available for research use only.
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Description

Interleukin-6 is a protein that in humans is encoded by the IL-6 gene. It is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin-6 is one of the most important mediators of fever and of the acute phase response. It is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al.(1988) assigned the IL-6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.(1988) mapped the IL6 gene to chromosome 7p15.

Application Notes

The stated application concentrations are suggested starting points. Titration of the IL-6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 195-212 (LRSFKEFLQSSLRALRQM) were used as the immunogen for this IL-6 antibody.

Storage

After reconstitution, the IL-6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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