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Home >> Antibodies >> IL-4 Antibody

IL-4 Antibody (F49086)

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  Catalog No Formulation Size Price (USD)  
Image F49086-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F49086-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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(Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot analysis of IL-4 antibody and mouse cerebellum lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.
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(Left) Western blot analysis of IL-4 antibody and WiDr lysate; (Right) Western blot analysis of IL-4 antibody and mouse cerebellum lysate. Expected molecular weight: 14-20 kDa depending on glycosylation level.
IL-4 antibody IHC analysis in formalin fixed and paraffin embedded tonsil
IL-4 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.-
Availability 1-3 business days
Species Reactivity Human, Mouse
Predicted Reactivity Primate
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P05112
Localization Secreted
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This IL-4 antibody is available for research use only.
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Description

IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.

Application Notes

Titration of the IL-4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 122-150 from the human protein was used as the immunogen for this IL-4 antibody.

Storage

Aliquot the IL-4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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