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Home >> Antibodies >> IL-10 Antibody

IL-10 Antibody (F51527)

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  Catalog No Formulation Size Price (USD)  
Image F51527-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F51527-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IL-10 antibody flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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IL-10 antibody flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human breast carcinoma stained with IL-10 antibody
Western blot analysis of IL-10 antibody and MDA-MB435 lysate. Predicted molecular weight ~20 kDa.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Primate
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P22301
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This IL-10 antibody is available for research use only.
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Description

The protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Application Notes

Titration of the IL-10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 27-53 from the human protein was used as the immunogen for this IL-10 antibody.

Storage

Aliquot the IL-10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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