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Home >> Antibodies >> IFNG Antibody / Interferon gamma

IFNG Antibody / Interferon gamma (RQ4575)

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  Catalog No Formulation Size Price (USD)  
Image RQ4575 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) placenta, 3) U-87 MG, 4) HL-60 and 5) U-937 lysate with IFNG antibody at 0.5ug/ml. Expected molecular weight: 19-24 kDa depending on glycosylation level.
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Western blot testing of human 1) HeLa, 2) placenta, 3) U-87 MG, 4) HL-60 and 5) U-937 lysate with IFNG antibody at 0.5ug/ml. Expected molecular weight: 19-24 kDa depending on glycosylation level.
Western blot testing of 1) rat brain, 2) rat lung, 3) mouse brain, 4) mouse ovary and 5) mouse HEPA 1-6 lysate with IFNG antibody at 0.5ug/ml. Expected molecular weight: 19-24 kDa depending on glycosylation level.
IHC staining of FFPE human sarcoma with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with IFNG antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P01579
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
ELISA (Capture) : 1-5ug/ml (human recombinant protein)
Limitations This IFNG antibody is available for research use only.
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Description

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Application Notes

Optimal dilution of the IFNG antibody should be determined by the researcher.

Immunogen

Amino acids Q24-G161 from the human protein were used as the immunogen for the IFNG antibody.

Storage

After reconstitution, the IFNG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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