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Home >> Antibodies >> IDH2 Antibody

IDH2 Antibody (R32113)

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  Catalog No Formulation Size Price (USD)  
Image R32113 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat heart, 2) rat liver, 3) mouse NIH3T3, 4) human SW620, 5) HeLa, 6) MCF7 and 7) 22RV1 lysate with IDH2 antibody. Predicted molecular weight: 45-51 kDa (two isoforms).
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Western blot testing of 1) rat heart, 2) rat liver, 3) mouse NIH3T3, 4) human SW620, 5) HeLa, 6) MCF7 and 7) 22RV1 lysate with IDH2 antibody. Predicted molecular weight: 45-51 kDa (two isoforms).
IHC testing of FFPE human intestinal cancer tissue with IDH2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse intestine with IDH2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat intestine with IDH2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placental tissue with IDH2 antibody.
IHC testing of frozen mouse small intestine tissue with IDH2 antibody.
IHC testing of frozen rat small intestine tissue with IDH2 antibody.
ICC staining of FFPE human A549 cells with IDH2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
ICC staining of FFPE mouse Hepa 1-6 cells with IDH2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
ICC staining of FFPE human SW480 cells with IDH2 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE rat NRK cells with IDH2 antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with IDH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IDH2 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P48735
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunocytochemistry (FFPE) : 1-2ug/ml
Immunofluorescence : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This IDH2 antibody is available for research use only.
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Description

Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the IDH2 antibody should be determined by the researcher.

Immunogen

Amino acids KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR of human IDH2 were used as the immunogen for the IDH2 antibody.

Storage

After reconstitution, the IDH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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