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Home >> Antibodies >> ICAM1 Antibody / CD54

ICAM1 Antibody / CD54 (R31451)

  Catalog No Formulation Size Price (USD)  
Image R31451 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of ICAM1 antibody and rat samples 1: thymus; 2: spleen; 3: PC12; 4: NRK; 5: RH35 lysate. The expected molecular weight of this glycoprotein is 75~115 kDa.
IHC-P: ICAM1 antibody testing of mouse intestine tissue
IHC-P: ICAM1 antibody testing of mouse lung tissue
IHC-P testing of mouse spleen tissue
IHC-P testing of rat intestine tissue
IHC-P testing of rat spleen tissue
Western blot testing of 1) rat NRK, 2) mouse spleen, 3) mouse liver, 4) mouse kidney and 5) mouse ANA-1 cell lysate with ICAM1 antibody. Expected molecular weight: 75-115 kDa.
IHC staining of FFPE mouse liver tissue with ICAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat liver tissue with ICAM1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 15894
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This ICAM1 antibody is available for research use only.
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Description

ICAM1 is a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. It binds to leukocyte function-associated antigen (LFA-1) on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.

Application Notes

Titration of the ICAM1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Mouse partial recombinant protein (AA 198-537) was used as the immunogen for this ICAM1 antibody.

Storage

After reconstitution, the ICAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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