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Home >> Antibodies >> HSP90AA1 Antibody / HSP90 alpha

HSP90AA1 Antibody / HSP90 alpha [clone HSP90AA1/7426] (V4367)

  Catalog No Formulation Size Price (USD)  
Image V4367-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4367-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4367SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human spleen tissue with HSP90AA1 antibody (clone HSP90AA1/7426). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free HSP90AA1 antibody (clone HSP90AA1/7426) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name HSP90AA1/7426
Purity Protein A/G affinity
UniProt P07900
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This HSP90AA1 antibody is available for research use only.
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Description

The heat shock response was first described for Drosophila salivary gland cells and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial chicken and mammalian cells, and have been subsequently studied in other organisms. A series of proteins including HSP 90, HSP 70, HSP 20-30 and ubiquitin are induced by insults such as temperature shock, chemicals and other environmental stress. A major function of HSP 90 and other HSPs is to act as molecular chaperones. HSP 90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP 56 or Cyclophilin D, forming an aporeceptor comiplex. HSP 90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an apo-receptor complex with estrogen and androgen receptors.

Application Notes

Optimal dilution of the HSP90AA1 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 500-700) from the human protein was used as the immunogen for the HSP90AA1 antibody.

Storage

Aliquot the HSP90AA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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