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Home >> Antibodies >> HSP70-2 Antibody / HSPA1B

HSP70-2 Antibody / HSPA1B [clone HSPA1B/7625] (V5117)

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  Catalog No Formulation Size Price (USD)  
Image V5117-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5117-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5117SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human breast carcinoma tissue with HSP70-2 antibody (clone HSPA1B/7625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast carcinoma tissue with HSP70-2 antibody (clone HSPA1B/7625). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human T-47D & HEK293 cell lysates using HSP70-2 antibody (clone HSPA1B/7625). Expected molecular weight ~70 kDa.
SDS-PAGE analysis of purified, BSA-free HSP70-2 antibody (clone HSPA1B/7625) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name HSPA1B/7625
Purity Protein A/G affinity
UniProt P0DMV9
Localization Cytoplasm, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Western blot : 1-2ug/ml
Limitations This HSP70-2 antibody is available for research use only.
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Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1.The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Application Notes

Optimal dilution of the HSP70-2 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 400-600) from the human protein was used as the immunogen for the HSP70-2 antibody.

Storage

Aliquot the HSP70-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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