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Home >> Antibodies >> HSP60 Antibody / HSPD1

HSP60 Antibody / HSPD1 [clone SRPR60] (V7117)

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  Catalog No Formulation Size Price (USD)  
Image V7117-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7117-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7117SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7117IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone SRPR60, green) and Reddot nuclear stain (red).
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Immunofluorescent staining of MeOH-fixed human HeLa cells with HSP60 antibody (clone SRPR60, green) and Reddot nuclear stain (red).
IHC testing of FFPE human bladder carcinoma and HSP60 antibody (clone SRPR60). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human lung carcinoma and HSP60 antibody (clone SRPR60). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa lysate with HSP60 antibody (clone SRPR60). Predicted molecular weight: ~60 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SRPR60
Purity Protein G affinity chromatography
UniProt P10809
Localization Cytoplasm (mitochondria)
Applications Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This HSP60 antibody is available for research use only.
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Description

HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]

Application Notes

Optimal dilution of the HSP60 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human HSPD1 protein was used as the immunogen for the HSP60 antibody.

Storage

Store the HSP60 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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