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Home >> Antibodies >> HSP60 Antibody / HSPD1

HSP60 Antibody / HSPD1 (R30272)

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  Catalog No Formulation Size Price (USD)  
Image R30272 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of HSP60 antibody and Lane 1: rat brain; 2: rat liver; 3: CEM; 4: HeLa; 5: SMMC-7721; 6: COLO320 cell lysate
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Western blot testing of HSP60 antibody and Lane 1: rat brain; 2: rat liver; 3: CEM; 4: HeLa; 5: SMMC-7721; 6: COLO320 cell lysate
IHC-P: HSP60 antibody testing of rat intestine tissue
ICC: SMMC-7721 cell
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt P63039
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This HSP60 antibody is available for research use only.
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Description

Heat shock 60KD protein is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. It can downregulate adaptive immune responses by upregulating Tregs innately throughTLR2signaling.

Application Notes

Variations in secondary/substrate sensitivities and test protocols may require the HSP60 antibody to be titrated for optimal performance.

Immunogen

An amino acid sequence from the C-terminus of rat HSP60 (YDAMLGDFVNMVEK) was used as the immunogen for this HSP60 antibody.

Storage

The lyophilized HSP60 antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

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