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Home >> Antibodies >> HSP27 Antibody

HSP27 Antibody [clone G3.1] (V2134)

  Catalog No Formulation Size Price (USD)  
V2134-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2134-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2134SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2134IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human breast carcinoma (10X) stained with HSP27 antibody (G3.1).
IHC testing of breast carcinoma (20X) stained with HSP27 antibody (G3.1).
IHC testing of FFPE human prostate carcinoma and HSP27 antibody.
SDS-PAGE Analysis of Purified, BSA-Free HSP27 Antibody (clone G3.1). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human, Chimpanzee, Sheep, Rat, Mouse, and Chicken. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name G3.1
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P04792
Localization Predominantly cytoplasmic with some nuclear
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Western blot : 0.25-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This HSP27 antibody is available for research use only.

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Description

This antibody recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). HSP27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for HSP27, especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.

Storage

Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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