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Home >> Antibodies >> HLA-A Antibody

HLA-A Antibody (R31867)

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  Catalog No Formulation Size Price (USD)  
Image R31867 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 419
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Western blot testing of 1) human U87, 2) human HepG2 and 3) mouse brain lysate with HLA-A antibody. Expected/observed molecular weight ~41 kDa.
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Western blot testing of 1) human U87, 2) human HepG2 and 3) mouse brain lysate with HLA-A antibody. Expected/observed molecular weight ~41 kDa.
IHC testing of FFPE human intestinal cancer with HLA-A antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat intestine with HLA-A antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P01892
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This HLA-A antibody is available for research use only.
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Description

HLA-A, namely HLA A, is a group of human leukocyte antigens (HLA) that are coded for by the HLA-A locus, which is located at human chromosome 6p21.3. HLA is simply the major histocompatibility complex (MHC) specific to humans. HLA-A is one of three major types of human MHC class I cell surface receptors. The others are HLA-B and HLA-C. And it is critical to the cytotoxic t-cell controlled immune response to viruses and other intracellular pathogens. Because each HLA-A gene has a high affinity for slightly different peptides, certain HLA-As are associated with increased risk, more rapid progression, and/or increased severity of many diseases. For similar reasons, HLA-A matching is essential to successful tissue transplants.

Application Notes

Optimal dilution of the HLA-A antibody should be determined by the researcher.

Immunogen

Amino acids EYWDGETRKVKAHSQTHRVDLGTLR of human HLA-A were used as the immunogen for the HLA-A antibody.

Storage

After reconstitution, the HLA-A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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