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  • Email: info@nsjbio.com
Home >> Antibodies >> HIF1 alpha Antibody

HIF1 alpha Antibody [clone HIF1A-84] (V2132)

  Catalog No Formulation Size Price (USD)  
V2132-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2132-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2132SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name HIF1A-84
Purity Protein G purified antibody
Buffer 1X PBS, pH 7.4
Gene ID 3091 (Human)
Localization Nuclear, possible cytoplasmic
Applications Flow Cytometry : 0.5-1ug/million cells
Immunofluorescence : 0.5-1ug/ml
Limitations This HIF1 alpha antibody is available for research use only.

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Description

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (alpha and beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIFs regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HIF1 alpha antibody to be titered up or down for optimal performance.

Immunogen

Recombinant human HIF1 alpha was used as the immunogen for this antibody.

Storage

Store the HIF1 alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Hypoxia inducible factor 1 alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS superfamily 1 (MOP1); PAS domain-containing protein 8 (PASD8), HIF1 alpha antibody

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