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  • Email: info@nsjbio.com
Home >> Antibodies >> HIF1 alpha Antibody

HIF1 alpha Antibody (F48865)

  Catalog No Formulation Size Price (USD)  
F48865-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 299

F48865-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 125
Western blot analysis of HIF1 alpha antibody and CHO lysate. Routinely observed molecular weight: 100~120 kDa.
HIF1 alpha antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma.
Fluorescent confocal image of HeLa cell stained with HIF1 alpha antibody at 1:25. HIF1A immunoreactivity is localized to the cytoplasm and nucleus.
Availability 1-3 business days
Species Reactivity Human, Hamster
Predicted Reactivity Bovine
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q16665
Localization Nuclear, possible cytoplasmic
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This HIF1 alpha antibody is available for research use only.

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Description

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This protein encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.

Application Notes

Titration of the HIF-1 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for this HIF1 alpha antibody.

Storage

Aliquot the HIF1 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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