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Home >> Antibodies >> Recombinant HER2 Antibody / Extracellular region

Recombinant HER2 Antibody / Extracellular region [clone ZR5] (V8632)

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  Catalog No Formulation Size Price (USD)  
Image V8632-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8632-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8632SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ZR5). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5); Red=isotype control, Blue= HER2 antibody.
Immunofluorescent staining of PFA-fixed human MCF7 cells with HER2 antibody (clone ZR5, green) and Phalloidin (red).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit
Clone Name ZR5
Purity Protein A affinity chromatography
UniProt P04626
Localization Cell surface
Applications Flow cytometry : 1-2ug/million cells
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant HER2 antibody is available for research use only.
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Description

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

Optimal dilution of the recombinant HER2 antibody should be determined by the researcher.

Immunogen

A recombinant protein encoding the extracellular domain of human c-erbB2 was used as the immunogen for the recombinant HER2 antibody.

Storage

Store the recombinant HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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