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Home >> Antibodies >> Recombinant ErbB2 Antibody / HER2 / Extracellular region

Recombinant ErbB2 Antibody / HER2 / Extracellular region [clone rERBB2/6637] (V4468)

  Catalog No Formulation Size Price (USD)  
Image V4468-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4468-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4468SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recmousemono
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IHC staining of FFPE human breast carcinoma tissue stained with recombinant ErbB2 antibody (clone rERBB2/6637) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue stained with recombinant ErbB2 antibody (clone rERBB2/6637) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC testing of FFPE human brain tissue with recombinant ErbB2 antibody (clone rERBB2/6637) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rERBB2/6637
Purity Protein A/G affinity
UniProt P04626
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This recombinant ErbB2 antibody is available for research use only.
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Description

Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This mAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Application Notes

Optimal dilution of the recombinant ErbB2 antibody should be determined by the researcher.

Immunogen

Recombinant protein encoding the extracellular domain of the human protein was used as the immunogen for the recombinant ErbB2 antibody.

Storage

Aliquot the recombinant ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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