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Home >> Antibodies >> HDGF Antibody

HDGF Antibody (R32600)

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  Catalog No Formulation Size Price (USD)  
Image R32600 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat liver and 2) human 22RV1 (prostate carcinoma) lysate with HDGF antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa but routinely observed at ~38 kDa.
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Western blot testing of 1) rat liver and 2) human 22RV1 (prostate carcinoma) lysate with HDGF antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa but routinely observed at ~38 kDa.
IHC testing of FFPE human lung carcinoma with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human intestinal carcinoma with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE mouse liver tissue with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat liver tissue with HDGF antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with HDGF antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with HDGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HDGF antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P51858
Localization Nuclear, cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Immunofluorescence (FFPE) : 2-4ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This HDGF antibody is available for research use only.
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Description

Hepatoma-derived growth factor (HDGF), also known as high mobility group protein 1-like 2 (HMG-1L2), is a protein that in humans is encoded by the HDGF gene. This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. And this gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Application Notes

Optimal dilution of the HDGF antibody should be determined by the researcher.

Immunogen

Amino acids 61-97 (KDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKA-human) were used as the immunogen for the HDGF antibody.

Storage

After reconstitution, the HDGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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