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Home >> Antibodies >> hCG alpha Antibody / Luteinizing Hormone alpha

hCG alpha Antibody / Luteinizing Hormone alpha [clone HCGa/53] (V2091)

  Catalog No Formulation Size Price (USD)  
V2091-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2091-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2091SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359

V2091IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 329
IHC testing of human placenta stained with hCG alpha antibody (HCGa/53). Note specific membrane staining.
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name HCGa/53
Purity Protein G purified monoclonal antibody
Buffer 1X PBS, pH 7.4
UniProt P01215
Localization Cytoplasmic, secreted
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Prediluted IHC only format : incubate for 30 min at RT (2)
Limitations This hCG alpha antibody is available for research use only.

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Description

This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).

Application Notes

Variations in protocols, secondaries and substrates may require the hCG alpha antibody to be titered for optimal performance.

1. No pretreatment required for FFPE section testing. HIER is not recommended.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for this hCG alpha antibody.

Storage

Store the hCG alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

FSHA; GPHA1; LHA; TSHA

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