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Home >> Antibodies >> H4 Antibody / Methyl-Lys20 specific

H4 Antibody / Methyl-Lys20 specific (F48612)

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  Catalog No Formulation Size Price (USD)  
Image F48612-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F48612-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from K562 cell line using H4 antibody diluted at 1:1000 for each lane.
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Western blot analysis of lysate from K562 cell line using H4 antibody diluted at 1:1000 for each lane.
IHC analysis of FFPE human lung carcinoma stained with H4 antibody
H4 antibody flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Dot blot analysis of H4 antibody (Methyl 2/methylation-specific). 50ng of methylation-peptide or Non methylation-peptide per dot were spotted.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P62805
Applications Western blot : 1:1000
Dot blot : 1:500
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This H4 antibody is available for research use only.
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Description

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

Application Notes

Titration of the H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids surrounding the K20 methylation site of the human protein were used as the immunogen for this Histone H4 antibody.

Storage

Aliquot the H4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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