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Home >> Antibodies >> Glycoprotein 36 Antibody / Gp36 / Podoplanin

Glycoprotein 36 Antibody / Gp36 / Podoplanin [clone PDPN/8875R] (V4701)

  Catalog No Formulation Size Price (USD)  
Image V4701-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4701-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4701SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated Recrabbitmono
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IHC staining of FFPE human prostate tissue with Glycoprotein 36 antibody (clone PDPN/8875R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Glycoprotein 36 antibody (clone PDPN/8875R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PDPN/8875R
Purity Protein A/G affinity
UniProt Q86YL7
Localization Cell surface
Applications ELISA (Order BSA-free format for coating) :
Immunofluorescence : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Glycoprotein 36 antibody is available for research use only.
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Description

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN), also called Glycoprotein 36 (Gp36). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endothelium as well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Application Notes

Optimal dilution of the Glycoprotein 36 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-150) from the human protein was used as the immunogen for the Glycoprotein 36 antibody.

Storage

Aliquot the Glycoprotein 36 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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