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Home >> Antibodies >> Glycophorin A Antibody

Glycophorin A Antibody [clone SGPA35] (V7130)

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  Catalog No Formulation Size Price (USD)  
Image V7130-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7130-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7130SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7130IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone SGPA35). Expected molecular weight: routinely observed at ~16/38 kDa.
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Western blot testing of human K562 cell lysate with Glycophorin A antibody (clone SGPA35). Expected molecular weight: routinely observed at ~16/38 kDa.
IHC testing of FFPE human placenta and Glycophorin A antibody (clone SGPA35). Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SGPA35
Purity Protein G affinity chromatography
UniProt P02724
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This Glycophorin A antibody is available for research use only.
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Description

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]

Application Notes

Optimal dilution of the Glycophorin A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human GPA protein was used as the immunogen for the Glycophorin A antibody.

Storage

Store the Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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