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Home >> Antibodies >> Glutamine Synthetase Antibody / GLUL

Glutamine Synthetase Antibody / GLUL [clone GLUL/6601] (V4755)

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  Catalog No Formulation Size Price (USD)  
Image V4755-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4755-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4755SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Western blot testing of human liver tissue lysate with Glutamine Synthetase antibody (clone GLUL/6601). Predicted molecular weight ~42 kDa.
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Western blot testing of human liver tissue lysate with Glutamine Synthetase antibody (clone GLUL/6601). Predicted molecular weight ~42 kDa.
Western blot testing of human Y79 cell lysate with Glutamine Synthetase antibody (clone GLUL/6601). Predicted molecular weight ~42 kDa.
IHC staining of FFPE human stomach tissue with Glutamine Synthetase antibody (clone GLUL/6601) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE dog liver with Glutamine Synthetase antibody (clone GLUL/6601). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human, Dog
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name GLUL/6601
Purity Protein A/G affinity
UniProt P15104
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Glutamine Synthetase antibody is available for research use only.
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Description

Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Application Notes

Optimal dilution of the Glutamine Synthetase antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the Glutamine Synthetase antibody.

Storage

Aliquot the Glutamine Synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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