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Home >> Antibodies >> GLO1 Antibody / Glyoxalase I

GLO1 Antibody / Glyoxalase I (RQ4238)

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  Catalog No Formulation Size Price (USD)  
Image RQ4238 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) COLO320, 5) 22RV1, 6) HepG2, 7) A431 and 8) U937 lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
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Western blot testing of human 1) HeLa, 2) placenta, 3) MCF7, 4) COLO320, 5) 22RV1, 6) HepG2, 7) A431 and 8) U937 lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
Western blot testing of rat 1) brain, 2) testis, 3) spleen, 4) thymus and mouse 5) brain, 6) testis, 7) thymus and 8) liver lysate with GLO1 antibody at 0.5ug/ml. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human lung cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human breast cancer tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with GLO1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q04760
Localization Cytoplasmic, nuclear, membrane
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This GLO1 antibody is available for research use only.
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Description

Lactoylglutathione lyase in humans is encoded by the GLO1 gene. The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.

Application Notes

Optimal dilution of the GLO1 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids A2-M184 was used as the immunogen for the GLO1 antibody.

Storage

After reconstitution, the GLO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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