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Home >> Antibodies >> GATA3 Antibody

GATA3 Antibody [clone 1259CT707.136.102] (F52445)

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  Catalog No Formulation Size Price (USD)  
Image F52445-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F52445-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot analysis of lysate from (1) CEM, (2) HL-60 and (3) SH-SY5Y cell lines using GATA3 antibody at 1:1000. Predicted molecular weight: 50 kDa
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Western blot analysis of lysate from (1) CEM, (2) HL-60 and (3) SH-SY5Y cell lines using GATA3 antibody at 1:1000. Predicted molecular weight: 50 kDa
Fluorescent image of MCF-7 cells stained with GATA3 antibody at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Flow cytometric analysis of MCF-7 cells using GATA3 antibody (green) and an isotype control of mouse IgG2b (blue); Ab was diluted at 1:25.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, k
Clone Name 1259CT707.136.102
Purity Purified
UniProt P23771
Applications Flow Cytometry : 1:25
Immunofluorescence : 1:25
Western blot : 1:1000
Limitations This GATA3 antibody is available for research use only.
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Description

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.

Application Notes

Titration of the GATA3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This GATA3 antibody was produced from a mouse immunized with a recombinant protein from human GATA3.

Storage

Aliquot the GATA3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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