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  • Email: info@nsjbio.com
Home >> Antibodies >> GAPDH Loading Control Antibody [Discontinued, view replacements]

GAPDH Loading Control Antibody [Discontinued, view replacements] (R31532)

  Catalog No Formulation Size Price (USD)  
R31532 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 285
Western blot testing of the GAPDH loading control antibody (0.5ug/ml) and Lane 1: rat brain; 2: HeLa; 3: COLO320. Predicted molecular weight ~36kDa.
Western blot testing of the GAPDH loading control antibody (0.5ug/ml) and recombinant human protein (0.5ng)
Availability Discontinued
Species Reactivity Human, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 2597
Localization Nuclear and cytoplasmic
Applications Western blot : 0.5-1ug/ml
Limitations This GAPDH loading control antibody is available for research use only.

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Description

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD).
GAPDH antibody is commonly used as a western blot loading control.

Application Notes

Titration of the GAPDH loading control antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids YSNRVVDLMAHMASKE were used as the immunogen for this GAPDH loading control antibody.

Storage

After reconstitution, the GAPDH loading control antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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