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Home >> Antibodies >> GAPDH Antibody / HRP Conjugate

GAPDH Antibody / HRP Conjugate [clone A-7] (RQ4477)

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  Catalog No Formulation Size Price (USD)  
Image RQ4477 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 429
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Western blot testing of 1) human Jurkat, 2) human A375, 3) human hippocampus, 4) human fetal liver, 5) monkey COS-1, 6) mouse RAW264.7, 7) mouse kidney, 8) rat PC-12 and 9) rat brain lysate with GAPDH antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
Western blot testing of 1) human Jurkat, 2) human A375, 3) human hippocampus, 4) human fetal liver, 5) monkey COS-1, 6) mouse RAW264.7, 7) mouse kidney, 8) rat PC-12 and 9) rat brain lysate with GAPDH antibody at 0.5ug/ml. Predicted molecular weight ~36 kDa.
Availability 1-2 weeks
Species Reactivity Human, Mouse, Rat
Format HRP Conjugate
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name A-7
Purity Protein A affinity
UniProt P04406
Localization Cytoplasm, Nucleus, Membrane
Applications Western blot : 1:5000-1:20000
Limitations This GAPDH antibody is available for research use only.
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Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. [UniProt]

Application Notes

Optimal dilution of the GAPDH antibody should be determined by the researcher.

Immunogen

A synthetic peptide from human GAPDH was used as the immunogen for the GAPDH antibody.

Storage

Store the GAPDH antibody at -20oC.

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