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Home >> Antibodies >> Galectin 3 Antibody

Galectin 3 Antibody (R31507)

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  Catalog No Formulation Size Price (USD)  
Image R31507 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) SiHa, 3) U-87 MG, 4) RT4, 5) HaCaT, 6) PC-3 and 7) T-47D cell lysate with Galectin 3 antibody. Predicted molecular weight ~26 kDa.
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Western blot testing of human 1) HeLa, 2) SiHa, 3) U-87 MG, 4) RT4, 5) HaCaT, 6) PC-3 and 7) T-47D cell lysate with Galectin 3 antibody. Predicted molecular weight ~26 kDa.
IHC-P: Galectin 3 antibody testing of human lung cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC-P: Galectin 3 antibody testing of human placenta tissue. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human U-2 OS cells with Galectin 3 antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE human SiHa cells with Galectin 3 antibody (green) at and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with Galectin 3 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Galectin 3 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Gene ID 3958
Localization Cytoplasmic, cell surface
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow cytometry : 1-3ug/10^6 cells
Limitations This Galectin 3 antibody is available for research use only.
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Description

Galectin 3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, it located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin 3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Galectin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 139-250) was used as the immunogen for this Galectin 3 antibody.

Storage

After reconstitution, the Galectin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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