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Home >> Antibodies >> Galectin 1 Antibody

Galectin 1 Antibody (R32842)

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  Catalog No Formulation Size Price (USD)  
Image R32842 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat stomach, 2) rat ovary, 3) rat testis, 4) mouse kidney and 5) human HeLa lysate with Galectin 1 antibody at 0.5ug/ml. Predicted molecular weight ~15 kDa.
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Western blot testing of 1) rat stomach, 2) rat ovary, 3) rat testis, 4) mouse kidney and 5) human HeLa lysate with Galectin 1 antibody at 0.5ug/ml. Predicted molecular weight ~15 kDa.
IHC testing of FFPE mouse small intestine tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse kidney tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat small intestine tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat kidney tissue with Galectin 1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P11762
Localization Cytoplasmic, secreted
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
ELISA (Capture; rat recombinant protein) : 0.1-0.5ug/ml (BSA-free format available)
Limitations This Galectin 1 antibody is available for research use only.
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Description

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.

Application Notes

Optimal dilution of the Galectin 1 antibody should be determined by the researcher.

Immunogen

A recombinant rat protein corresponding to amino acids A2-E135 was used as the immunogen for the Galectin 1 antibody.

Storage

After reconstitution, the Galectin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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