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Home >> Antibodies >> GAD67 Antibody / GAD1

GAD67 Antibody / GAD1 [clone GAD1/2563] (V3859)

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  Catalog No Formulation Size Price (USD)  
Image V3859-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3859-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3859SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC testing of FFPE human brain with GAD67 antibody (clone GAD1/2563). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human brain with GAD67 antibody (clone GAD1/2563). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GAD67 antibody (clone GAD1/2563). These results demonstrate the foremost specificity of the GAD1/2563 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free GAD67 antibody (clone GAD1/2563) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name GAD1/2563
Purity Protein G affinity chromatography
UniProt Q99259
Localization Cytoplasmic
Applications ELISA (order BSA/sodium azide-free format for coating) :
Flow cytometry : 1-2ug/10^6 cells
Western blot : 0.5-1ug/ml
Limitations This GAD67 antibody is available for research use only.
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Description

This mAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1). GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Application Notes

Optimal dilution of the GAD67 antibody should be determined by the researcher.

Immunogen

A portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.

Storage

Store the GAD67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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