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Home >> Antibodies >> GAD65 Antibody / GAD2

GAD65 Antibody / GAD2 [clone GAD2/6484] (V4087)

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  Catalog No Formulation Size Price (USD)  
Image V4087-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4087-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4087SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human thyroid tissue with GAD2 (GAD65) antibody (clone GAD2/6484). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human thyroid tissue with GAD2 (GAD65) antibody (clone GAD2/6484). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free GAD2 (GAD65) antibody (clone GAD2/6484) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name GAD2/6484
Purity Protein A/G affinity
UniProt Q05329
Localization Cytoplasm
Applications ELISA (Order BSA-free format for coating) :
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This GAD65 antibody is available for research use only.
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Description

This mAb recognizes a protein of 65kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Application Notes

Optimal dilution of the GAD65 antibody should be determined by the researcher.

Immunogen

A recombinant fragment of human GAD2/GAD65 protein (within amino acids 1-200) was used as the immunogen for the GAD65 antibody.

Storage

Aliquot the GAD65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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