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Home >> Antibodies >> G-CSF Antibody / Granulocyte-colony stimulating factor

G-CSF Antibody / Granulocyte-colony stimulating factor (R31928)

  Catalog No Formulation Size Price (USD)  
Image R31928 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE human rectum adenocarcinoma tissue with G-CSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung squamous cell carcinoma tissue with G-CSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human spleen tissue with G-CSF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HepG2, 3) MOLT4 and 4) MCF7 cell lysate with G-CSF antibody. Expected molecular weight ~22 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P09919
Localization Cytoplasm
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested)
Limitations This G-CSF antibody is available for research use only.
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Description

Granulocyte-colony stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. Functionally, it is a cytokine and hormone, a type of colony-stimulating factor, and is produced by a number of different tissues. The pharmaceutical analogs of naturally occurring G-CSF are called filgrastim and lenograstim. G-CSF also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils.

Application Notes

Optimal dilution of the G-CSF antibody should be determined by the researcher.

Immunogen

Amino acids 38-207 of human G-CSF were used as the immunogen for the G-CSF antibody.

Storage

After reconstitution, the G-CSF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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