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Home >> Antibodies >> FZD1 Antibody / Frizzled 1

FZD1 Antibody / Frizzled 1 (R32354)

  Catalog No Formulation Size Price (USD)  
Image R32354 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) 22RV1 and 2) 293 cell lysate with FZD1 antibody. Predicted/observed molecular weight ~71 kDa.
IHC testing of FFPE human prostate cancer tissue with FZD1 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q9UP38
Localization Cytoplasmic, membrane
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
Limitations This FZD1 antibody is available for research use only.
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Description

Frizzled-1 (Frizzled homolog 1) is a protein that in humans is encoded by the FZD1 gene. Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signalingproteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.

Application Notes

Optimal dilution of the FZD1 antibody should be determined by the researcher.

Immunogen

Amino acids YIAGFLLEDRVVCNDKFAEDGARTVAQG TKKE of human Frizzled 1 were used as the immunogen for the FZD1 antibody.

Storage

After reconstitution, the FZD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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