Flotillin 2 Antibody (R32227)

Immunofluorescent staining of FFPE human A431 cells with Flotillin 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

IHC testing of FFPE human breast cancer tissue with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE mouse intestine with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of FFPE rat kidney with Flotillin-2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

IHC testing of frozen mouse kidney tissue with Flotillin 2 antibody.

IHC testing of frozen rat kidney tissue with Flotillin 2 antibody.

IHC testing of frozen rat small intestine tissue with Flotillin 2 antibody.

Western blot testing of 1) rat liver, 2) rat lung, 3) mouse liver, 4) mouse lung, 5) mouse testis, 6) human placenta, 7) A375, 8) HeLa, 9) 22RV1 and 10) mouse NIH3T3 lysate with Flotillin 2 antibody. Predicted molecular weight: ~47 kDa.

Flow cytometry testing of human U-2 OS cells with Flotillin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Flotillin 2 antibody.

Flow cytometry testing of rat RH35 cells with Flotillin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Flotillin 2 antibody.