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Home >> Antibodies >> FITC Antibody

FITC Antibody [clone SPM395] (V3349)

  Catalog No Formulation Size Price (USD)  
Image V3349-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3349-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3349SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Availability 1-3 business days
Species Reactivity NA
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM395
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This FITC antibody is available for research use only.
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Description

This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this mAb is also suitable for the conversion of fluorescein-labeled detection into immuno-enzymatic assays.

Application Notes

Optimal dilution of the FITC antibody should be determined by the researcher.

Immunogen

FITC conjugated to KLH was used as the immunogen for this FITC antibody.

Storage

Store the FITC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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