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Home >> Antibodies >> Filaggrin Antibody

Filaggrin Antibody [clone FLG/1562] (V3331)

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  Catalog No Formulation Size Price (USD)  
Image V3331-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3331-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3331SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1562). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human skin with Filaggrin antibody (clone FLG/1562). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Filaggrin Antibody (clone FLG/1562). Confirmation of Integrity and Purity of the Antibody.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name FLG/1562
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt P20930
Localization Cytoplasmic
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Limitations This Filaggrin antibody is available for research use only.
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Description

Filaggrin (FLG) is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple Filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional Filaggrin molecules. Active Filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Application Notes

Optimal dilution of the Filaggrin antibody should be determined by the researcher.

Immunogen

Amino acids 198-288 of human FLG were used as the immunogen for this Filaggrin antibody.

Storage

Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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