FH Antibody | R34252 NSJ Bioreagents

	Catalog No	Formulation	Size	Price (USD)
	R34252-100UG	0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide	100 ug	429
Formulation

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Western blot testing of human kidney lysate with FH antibody at 0.01ug/ml. Predicted molecular weight: ~55/50 kDa (isoforms 1/2).

Western blot of HEK293 lysate overexpressing Fumarase probed with FH antibody (mock transfection in lane 1). Predicted molecular weight: ~55/50 kDa (isoforms 1/2).

Sandwich ELISA using the FH antibody at 1.5ug/ml as the detect.

IHC testing of FFPE human skeletal muscle tissue with FH antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

IHC testing of FFPE human heart tissue with FH antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Availability	1-3 business days
Species Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog
Format	Antigen affinity purified
Clonality	Polyclonal (goat origin)
Isotype	Goat Ig
Purity	Antigen affinity
UniProt	P07954
Gene ID	2271
Localization	Cytoplasmic, mitochondrial
Applications	Western blot : 0.01-0.1ug/ml IHC (FFPE) : 3.75ug/ml Enzyme ImmunoAssay : used as capture for recombinant protein (coat at 1-2ug/ml) ELISA (peptide) LOD : 1:64000
Limitations	This FH antibody is available for research use only.
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