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Home >> Antibodies >> FCGR1A Antibody / CD64

FCGR1A Antibody / CD64 [clone FCGR1A/7497] (V4724)

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  Catalog No Formulation Size Price (USD)  
Image V4724-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V4724-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V4724SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil tissue with FCGR1A antibody (clone FCGR1A/7497) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with FCGR1A antibody (clone FCGR1A/7497) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free FCGR1A antibody (clone FCGR1A/7497) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2, kappa
Clone Name FCGR1A/7497
Purity Protein A/G affinity
UniProt P12314
Localization Cell Membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This FCGR1A antibody is available for research use only.
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Description

CD64/FCGR1A is a surface glycoprotein with high affinity for monomeric IgG, is expressed constitutively on monocytes and macrophages, and can be induced in neutrophils subsequent to IFN-gamma stimulation. It plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64/FCGR1A has been shown to associate with signal transducing subunit of the high affinity IgE receptor.

Application Notes

Optimal dilution of the FCGR1A antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the FCGR1A antibody.

Storage

Aliquot the FCGR1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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