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Home >> Antibodies >> Fatty Acid Synthase Antibody

Fatty Acid Synthase Antibody [clone 497CT15.2.5] (F40374)

  Catalog No Formulation Size Price (USD)  
Image F40374-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F40374-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Fluorescent confocal image of HepG2 cells stained with Fatty Acid Synthase antibody. Alexa Fluor 488 secondary was used (green). FASN immunosignal localizes to the cytoplasm.
Fatty Acid Synthase antibody western blot analysis in mouse brain tissue lysate. Predicted molecular weight ~ 270 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgM
Clone Name 497CT15.2.5
Purity Purified
UniProt P49327
Localization Cytoplasmic
Applications Immunofluorescence : 1:200
Western blot : 1:100
Limitations This Fatty Acid Synthase antibody is available for research use only.
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Description

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Application Notes

Titration of the Fatty Acid Synthase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 942-973 from human FASN was used as the immunogen for this Fatty Acid Synthase antibody.

Storage

Aliquot the Fatty Acid Synthase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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