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Home >> Antibodies >> FAK Antibody / Focal Adhesion Kinase / PTK2

FAK Antibody / Focal Adhesion Kinase / PTK2 [clone 3D8-H11-E12] (F54023)

  Catalog No Formulation Size Price (USD)  
Image F54023-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of monkey COS7, human HUVEC, human A549 and human HeLa cell lysates with FAK antibody at 1:1000. FAK has numerous isoforms and is routinely observed in western blot between 80~125 kDa.
ICC/IF testing of HeLa cells, fixed fixed by anhydrous methanol at -20oC, with FAK antibody at 1:200.
Immunoprecipitation and western blot of HeLa cell lysate using the FAK antibody.
Availability 1-3 business days
Species Reactivity Human, Primate
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Clone Name 3D8-H11-E12
Purity Protein G affinity
UniProt Q05397
Applications Western blot : 1:1000
ICC/IF : 1:200
Immunoprecipitation : suitable
Limitations This FAK antibody is available for research use only.
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Description

Focal Adhesion Kinase, or FAK, is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the FAK antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant partial protein was used as the immunogen for this FAK antibody.

Storage

Store the FAK antibody at -20oC.

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