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Home >> Antibodies >> Elastase 3B Antibody / CELA3B

Elastase 3B Antibody / CELA3B [clone CELA3B/1257] (V3436)

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  Catalog No Formulation Size Price (USD)  
Image V3436-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3436-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3436SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE mouse pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone CELA3B/1257). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Elastase 3B Antibody (clone CELA3B/1257). Confirmation of Integrity and Purity of the Antibody.
Western blot of human PANC-1 cell lysate using Elastase 3B antibody (CELA3B/1257). Predicted molecular weight ~29 kDa.
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CELA3B/1257
Purity Protein G
UniProt P08861
Localization Cytoplasmic
Applications ELISA : 2-4ug/ml (order BSA/azide-free format)
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Elastase 3B antibody is available for research use only.
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Description

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

Application Notes

Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

1. FFPE staining requires

Immunogen

A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

Storage

Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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