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Home >> Antibodies >> Elafin Antibody / PI3

Elafin Antibody / PI3 (R32376)

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  Catalog No Formulation Size Price (USD)  
Image R32376 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human placenta lysate with Elafin antibody. Predicted molecular weight ~12 kDa (pre-form), observed here at ~25 kDa.
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Western blot testing of human placenta lysate with Elafin antibody. Predicted molecular weight ~12 kDa (pre-form), observed here at ~25 kDa.
IHC testing of FFPE human tonsil with Elafin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P19957
Localization Cytoplasmic
Applications Western blot : 0.1-0.5ug/ml
IHC (FFPE) : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (human protein tested); request BSA-free format for coating
Limitations This Elafin antibody is available for research use only.
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Description

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Application Notes

Optimal dilution of the Elafin antibody should be determined by the researcher.

Immunogen

Amino acids A61-Q117 from the human protein were used as the immunogen for the Elafin antibody.

Storage

After reconstitution, the Elafin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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