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Home >> Antibodies >> EGFR Antibody

EGFR Antibody [clone GFR450] (V2105)

  Catalog No Formulation Size Price (USD)  
V2105-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 329

V2105-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 129

V2105SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 359
IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).
FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).
Immunofluorescent staining of A431 cells with Alexa Fluor conjugated EGFR antibody (green) and DAPI (nuclear, blue).
SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone GFR450). Confirmation of Integrity and Purity of the Antibody.
Species Reactivity Human. Other species not known.
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name GFR450
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
Gene ID 1956
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT (1)
Flow Cytometry : 0.5-1ug/million cells in 0.1ml
Immunofluorescence : 1-2ug/ml
Limitations This EGFR antibody is available for research use only.

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Description

This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

The immunizing protein for this antibody was recombinant human EGFR.

Storage

Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

ERBB1; Errp; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor Tyrosine Protein Kinase; Urogastrone; wa2; Wa5, EGFR antibody, ERBB1 antibody

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