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Home >> Antibodies >> EGF Receptor Antibody / EGF Receptor

EGF Receptor Antibody / EGF Receptor [clone EGFR/4616] (V5196)

  Catalog No Formulation Size Price (USD)  
Image V5196-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 519
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V5196-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 229
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V5196SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human placental tissue with EGF Receptor antibody (clone EGFR/4616). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free EGF Receptor antibody (clone EGFR/4616) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name EGFR/4616
Purity Protein A/G affinity
UniProt P00533
Localization Cell Surface,
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This EGF Receptor antibody is available for research use only.
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Description

This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Optimal dilution of the EGF Receptor antibody should be determined by the researcher.

Immunogen

A His-tagged human protein fragment was used as the immunogen for the EGF Receptor antibody.

Storage

Aliquot the EGF Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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