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Home >> Antibodies >> E-Cadherin Antibody / CDH1

E-Cadherin Antibody / CDH1 [clone ECD1-2] (V7230)

  Catalog No Formulation Size Price (USD)  
Image V7230-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7230-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7230SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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Immunofluorescent staining of human MCF7 cells with E-Cadherin antibody (clone ECD1-2, green) and Reddot nuclear stain (red).
IHC testing of FFPE human skin with E-Cadherin antibody (clone ECD1-2). HIER: steam sections in pH6 citrate buffer for 20 min.
IHC testing of FFPE human placenta with E-Cadherin antibody (clone ECD1-2). HIER: steam sections in pH6 citrate buffer for 20 min.
Western blot testing of 1) partial recombinant protein and 2) human stomach lysate using E-Cadherin antibody at 0.5ug/ml. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ECD1-2
Purity Protein G affinity chromatography
UniProt P12830
Localization Cytoplasmic, membranous
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Immunofluorescence : 2-4ug/ml
Limitations This E-Cadherin antibody is available for research use only.
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Description

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. [UniProt]

Application Notes

Optimal dilution of the E-Cadherin antibody should be determined by the researcher.

Immunogen

A full length recombinant human protein was used as the immunogen for the E-Cadherin antibody.

Storage

Store the E-Cadherin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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