- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Dopamine Beta-Hydroxylase Antibody / DBH [clone DBH/7222] (V5312)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
V5312-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 519 | |
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V5312-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 229 | |
|
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V5312SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 519 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2, kappa |
| Clone Name | DBH/7222 |
| Purity | Protein A/G affinity |
| UniProt | P09172 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This Dopamine Beta-Hydroxylase antibody is available for research use only. |
|
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Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to noradrenaline in the biosynthesis of catecholamines. DBH is selectively expressed in noradrenergic and adrenergic neurons, as well as in neuroendocrine cells, and it serves as a specific protein marker for noradrenergic processes. The active form of DBH is a homotetramer, which is found in the lumen of synaptic vesicles of corresponding nerve cells, where it localizes to both the membrane and cytosol. DBH is induced by nerve growth factor and Insulin growth factor-1 and is regulated by intracellular second messengers protein kinase A, cyclic AMP, diacyl glycerol and Ca2+. Expression of DBH is transcriptionally mediated by Sp1, CREB and AP-1 proteins including c-Fos, c-Jun and JunD.
Optimal dilution of the Dopamine Beta-Hydroxylase antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the Dopamine Beta-Hydroxylase antibody.
Aliquot the Dopamine Beta-Hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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