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Home >> Antibodies >> DOG1 Antibody

DOG1 Antibody [clone DG1/1485] (V3334)

  Catalog No Formulation Size Price (USD)  
Image V3334-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3334-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3334SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Microvalidated
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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1485). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using DOG1 antibody (clone DG1/1485). These results demonstrate the foremost specificity of the DG1/1485 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free DOG1 antibody (clone DG1/1485) as confirmation of integrity and purity.
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name DG1/1485
Purity Protein G affinity chromatography
Buffer 1X PBS, pH 7.4
UniProt Q5XXA6
Gene ID 55107
Localization Cell Surface and Cytoplasmic
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This DOG1 antibody is available for research use only.
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Description

DOG1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.

Immunogen

A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.

Storage

Store the DOG1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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