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Home >> Antibodies >> DJ-1 Antibody / PARK7

DJ-1 Antibody / PARK7 (R31721)

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  Catalog No Formulation Size Price (USD)  
Image R31721 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of DJ-1 antibody and human samples 1: PANC; 2: U-2 0S; 3: SMMC-7721; 4: HeLa lysate. Predicted/observed molecular weight ~20 kDa.
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Western blot testing of DJ-1 antibody and human samples 1: PANC; 2: U-2 0S; 3: SMMC-7721; 4: HeLa lysate. Predicted/observed molecular weight ~20 kDa.
IHC-P: DJ-1 antibody testing of human pancreatic cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: DJ-1 antibody testing of mouse pancreas tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: DJ-1 antibody testing of rat pancreas tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of human A549 cells with DJ-1 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Gene ID 11315
Localization Cytoplasmic & Nuclear
Applications Western blot : 0.5-1ug/ml
IHC (FFPE) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations DJ-1 antibody is available for research use only.
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Description

Parkinson disease 7, also known as Protein deglycase DJ-1, is a protein which in humans is encoded by the PARK7 gene. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It is also involved in tumorigenesis and in maintaining mitochondrial homeostasis. DJ-1 may function as a redox-sensitive chaperone, as a sensor foroxidative stress, and it apparently protects neurons against oxidative stress and cell death. It has been found that DJ-1 mutations that impair transcriptional coactivator function can render dopaminergic neurons vulnerable to apoptosis and may contribute to the pathogenesis of Parkinson disease.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the DJ-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Human partial recombinant protein (AA 2-189) was used as the immunogen for this DJ-1 antibody.

Storage

After reconstitution, the DJ-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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